Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences Tris(hydroxymethyl)aminomethane ACS reagent, >=99.8% | 77-86-1 | MFCD00004679 | 100G
Tris(hydroxymethyl)aminomethane ACS reagent, >=99.8% | Purity: >=99.8%; 99.8-100.1% | Mol Wt: 121.14 | 77-86-1 | MFCD00004679 | 100G
Medchemexpress LLC 4-Morpholineethanesulfonic acid, hydrate | 1266615-59-1 | 99.9% | 25 G
4-Morpholineethanesulfonic acid, hydrate | 1266615-59-1 | 99.9% | 25 G
Ethos Biosciences Buffer Diluent phosphate concentrate 1/EA
Dilurex 10X PBS is a concentrated solution of phosphate buffered saline intended as a stock solution Save space by keeping 10x concentrate and dilute to 1x pH 7 4 working concentration as needed by mixing 1 part 10X PBS with 9 parts distilled water Skip the tedium of weighing powders and stocking raw materials that can expire Our PBS is convenient ready to use and will save you hours in the lab letting you focus on breakthroughs rather than buffer prep Thousands of researchers trust Ethos Biosciences 10X PBS for its proven track record in top-tier publications and protocols - upgrade your productivity today
Sigma Aldrich Fine Chemicals Biosciences PIPES | 5625-37-6 | MFCD00006159 | 100g
PIPES | 99% | MW: 307.32 | 5625-37-6 | MFCD00006159 | 100g
Sigma Aldrich Fine Chemicals Biosciences PIPES BioXtra, for molecular biology, >=99.5% (T) | 5625-37-6 | MFCD00006159 | 50G
PIPES BioXtra, for molecular biology, >=99.5% (T) | Purity: >=99.5% (T) | Mol Wt: 302.37 | 5625-37-6 | MFCD00006159 | 50G
Bio Rad Laboratories XT MOPS RUNNING BUFFER 500ML
Extended range protein standard, 500ul, 50 applications. Product contains 12 bands from 2kD to 250kD. 250, 150, 100, 50, 37, 20, 15, 10 & 5 kD are blue, 75, 25 and 2kD are pink.
Medchemexpress LLC Glycine, N-(2-amino-2-oxoethyl)-N-(carboxymethyl)-, sodium salt (1:2) | 41689-31-0 | 98.0% | 25 G
ADA disodium is a buffer salt derived from ADA (adenosine deaminase) and is widely used in biochemical research, especially in the preparation of enzyme buffers.
- Derived from adenosine deaminase
- Used in biochemical research
- Ideal for enzyme buffer preparation
Medchemexpress LLC Bicine | 150-25-4 | 98.7% | 25 G
Bicine is a buffer utilized in physiological research. It is a derivative of the amino acid glycine that enhances the sensitivity of guanosine enzyme histochemical detection.
- Buffer utilized in physiological research
- Derivative of the amino acid glycine
- Enhances the sensitivity of guanosine enzyme histochemical detection
Abcam Salsolinol hydrochloride, Dopamine derivative, 500MG
MW 215.68 g/mol, Purity >99%. Dopamine (ab120565) derivative. Putative endogenous opioid system-activating factor. Antagonizes L-DOPA-mediated hyperactivity. Shows central effects in vivo. .
The product is subject to the following: Abcam Restricted Use Statement
Sigma Aldrich Fine Chemicals Biosciences BES >=99.0% (titration) | 10191-18-1 | MFCD00007533 | 25G
BES >=99.0% (titration) | Purity: >=99.0% (titration) | Mol Wt: 213.25 | 10191-18-1 | MFCD00007533 | 25G
Medchemexpress LLC 2-Morpholinoethanesulphonic acid monohydrate (Standard) | 145224-94-8 | 99.95% | 50 MG
MES monohydrate (Standard) is the analytical standard of MES (monohydrate), intended for research and analytical applications. MES (2-Morpholinoethanesulphonic acid) is an amphoteric ion buffer with a buffer capacity ranging from pH 5.5-7.0. As a Good's buffer, it is widely used in biochemistry and molecular biology experiments.
- Used for research and analytical applications.
- Amphoteric ion buffer.
- Buffer capacity ranging pH 5.5-7.0.
- Widely used in biochemistry and molecular biology experiments.
- Suitable for cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Commonly used in qualitative, quantitative, and methodological research experiments in HPLC, GC, and MS.
Medchemexpress LLC L-Adenosine | 3080-29-3 | 99.8% | 1 ML
L-Adenosine is a metabolically stable enantiomeric analog and a potential probe. It exhibits weakly inhibitory adenosine deaminase (ADA) activity with a Ki value of 385 μM. This compound can be utilized for research into adenosine uptake and accumulation.
- Metabolically stable enantiomeric analog.
- Potential probe for research.
- Weakly inhibits adenosine deaminase activity.
- Can be used for adenosine uptake research.
- Can be used for adenosine accumulation research.