Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences BIS-TRIS propane BioXtra 925G
REAGENT BIS-TRIS propane BioXtra 925G
Sigma Aldrich Fine Chemicals Biosciences Phosphate Buffered Saline1L
Phosphate Buffered Saline (PBS) is routinely used as a wash buffer in Western blot and immunoprecipitation (IP) procedures as well as during sample preparation of cell lysates for use in general molecular biology applications.
Sigma Aldrich Fine Chemicals Biosciences PIPES sodium salt >=99% (titration) | 10010-67-0 | MFCD00065472 | 100G
PIPES sodium salt >=99% (titration) | Purity: >=99% (titration) | Mol Wt: 324.35 | 10010-67-0 | MFCD00065472 | 100G
Electron Microscopy Sciences Phosphate Buffered Saline, 2X 1 GL
PBS is commonly used in biochemistry. It is a salty solution containing sodium chloride, potassium chloride, sodium phosphate and potassium phosphate. PBS is isotonic and non-toxic to cells. 1X PBS final concentration is 8.0 g/L NaCl, 0.2 g/L KCl, 1.42 g/L Na2HPO4, 0.24 g/L KH2PO4 and pH 7.4.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Phosphate Bufer500ML
Dulbecco Phosphate Buffered Saline (DPBS) is a balanced salt SOLUTIONon.
PLANT CELL TECHNOLOGY 1X Phosphate Buffered Saline Solution, pH 7.4, without Calcium and Magnesium, Buffer Solution, Mod. 6, Clear Liquid, Sterile, 500mL bottle.
1X PBS Solution Mod. 6, pH 7.4, is a sterile buffer used in mammalian cell culture to maintain physiological pH during washing, dilution, or reagent preparation. Packaged in a clear 500mL bottle. Ready-to-use and stable under recommended conditions. Ideal for research labs, tissue culture, and molecular biology workflows.
